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When the mixture, then, was merely potential, the things that were separated off moved similarly from every side towards the centre.
The mixture then was added with 10 µL of 1 M IAA and incubated for 40 min in darkness at room temperature.
The mixture then was dialysed overnight against the buffer B (20 mM Tris-HCl pH 7.0, 75 mM NaCl, 10% glycerol (v/v), 1 mM β-mercaptoethanol, 20 mM MgCl2, 0.05% NaN3 (w/v), 1 µg/ml PD 0305970).
SDS was removed from the LMW protein samples by tricholoroacetic acid (TCA) precipitation, in which the concentrated proteins/peptides were incubated with an equal volume of 10% TCA (w/v) on ice for 1 hour and the mixture then was centrifuged at 15,000x g for 30 minutes at 4°C.
The reaction mixture then was aspirated into the measuring cell, where the microparticles were captured magnetically upon the surface of the electrode.
This mixture then was extracted using SPE well plates and analyzed by reverse-phase HPLC/MS/MS (LLOQ, 50 ng/mL for the homogenate).
Similar(51)
The mixtures then were dried using a vacuum oven method.
If food processor is used, mixture must then be sieved to remove skins.
The mixture was then allowed to cool and then filtered.
The mixture was then assessed with 1 × PK reaction buffer.
This mixture was then fed to the fixed bed reactor.
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