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Providing adequate changes of variables, the problem was shown to amount to a mixture study with constraints placed on each factor.
A trend to slightly higher acetaldehyde yields (by up to 23%, nicotine-based, not dependent on sugar application level) was also observed in another ingredient mixture study with sugar application levels up to 10.5% (Baker et al., 2004b).
A trend to increased acrolein yields (up to 24%, nicotine-based, independent of the sugar application level) was also observed in another ingredient mixture study with sugar application levels of up to 10.5% (Baker et al., 2004b).
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Mixture studies with TB and the model ER agonists, 17α-ethinylestradiol and bisphenol A, also showed inhibition of tubercle formation in the females, but unlike the AR antagonists, the estrogens markedly induced synthesis of vitellogenin (VTG: egg yolk protein), particularly in males.
In mixture studies with a 1∶1 ratio by weight of DNA (n = 27 reactions), there was evidence of some cross-reactivity with mean amount of target bacterial DNA detected of 160% (range 107 268%).
However, in mixture studies with 1∶100 ratio of H. influenzae to H. parainfluenzae (n = 9 reactions), the mean amount of target bacteria detected was 118% (range 98 140%) suggesting low levels of amplification of H. parainfluenzae (Tables S2 and S3).
Good agreement was obtained between calculated and measured ignition delay times in the mixtures studied with and without the discharge.
The mixture study was conducted with serial dilutions of the mixture (n = 12/dose).
Predictions based on the responses seen with single agents run in parallel with the mixture study (Table 1, study 4) produced lower effect doses in the median- and high-effect range, in better agreement with the observed results.
Mixture studies conducted with 9a and PS (1) suggest that stimulation is occurring via similar modes of activation, demonstrating a relative lack of specificity for recognition of the allo-configuration (i.e., 5α) in sea lamprey olfaction.
The analyses for single compounds were carried out using effect data pooled from the initial dose response studies (Table 1, studies 1 3) and the repeat experiments run concurrently with the mixture study (Table 1, study 4).
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