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Compared to the mixture solutions applied in the first and third steps, more mixture solution applied in the second step was transported to the deep soil layer by macropores, corresponding to obvious peaks of soil water content and NO−3 concentration distributions observed in the deep soil layer in Plot 1.
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To simulate the salivary contamination in the bonding procedure in clinical situations, the polymer solution in water/methanol mixture was applied to the bonding dentin surface treated by artificial saliva, and then the AE adhesive resin was added.
Therefore, E-rGO, instead of C-rGO, was applied to detect DA in the mixture solution in the following experiment.
The liposome mixture was applied to the column and eluted with the equilibration solution.
The labeled DNA hybridization mixture was applied to the microarray using lifterslip coverslips and hybridization was carried out in a Genemachines Hybchamber (Genomic Solutions, Ann Arbor, MI, USA) for 14 18 hours at 60°C in a water bath.
Four minutes after the addition of RbCl, a 200-μl sample of the reaction mixture was applied to the Dowex column, which was then washed with 2 ml of an ice-cold solution that was collected in scintillation vials.
For negative stain analysis, aliquots of the reaction mixture were applied to carbon-Formvar coated 400 MESH copper grids and stained with a 2% (w/v) uranyl acetate solution.
Chromatograms of six replicas of the laboratory mixture solution were obtained and the effect of each deliberate change was evaluated by applying system suitability parameters and calculating value of RSD for each deliberate change.
NMS, nutritive mixture solution.
Another laboratory mixture solution was centrifuged (unfiltered).
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