The reaction mixtures were resolved in a 10% polyacrylamide gel in TBE buffer (89 mM Tris borate, pH 8.0, 2 mM EDTA).
PCR reaction mixtures were resolved in 1% (w/v) agarose gels and PCR products were purified by means of Wizard SV gel & PCR Clean Up kits (Promega).
This approach optimally determined a set of conditions in which cholesteryl esters were fully separated in standard mixtures and better resolved in a complex biological fluid in a timely fashion.
For example, a binary mixture could be successfully resolved in 0.8 ms using chip-based CE separation using a field strength of 53 kV/cm, with an analysis time of several orders of magnitudes less than conventional CE (Jacobson et al., 1998).
The eluates were pooled, dried under a stream of nitrogen, and resolved in a mixture of acetonitrile and water.
A large number of studies have demonstrated that the amino acids mixtures derivatized with OPA can be resolved in a relatively short period of time by HPLC, using reverse phase C-18 columns and a elution gradient (Cereser et al. 2001; Hanczko et al. 2007; Molnar-Perl 2011).
"When I record a scene, my aim is to create a mixture of plain information and riddles, so that not everything is resolved in the image.
