Sentence examples for mixture prepared directly from inspiring English sources

Transient transfection of RD cells was performed in 6-well plate format using Lipofectamine 2000 according to manufacturer's instructions with minor modification: 600.000 cells were added to the transfection mixture prepared directly in the cell culture plate.

The reaction mixtures were prepared directly in cuvettes using a 0.1 M sodium phosphate buffer, pH 7.0, 1 m M EDTA, and 0.33 m M DTT with 10 μ M enzyme in a final volume of 0.75 ml.

Glass also may be prepared directly from a gas.

The DNA template was prepared directly from two colonies of each strain suspended in 50 µl of double-distilled water and heated to 96°C for 10 min prior to the addition of the reaction mixture.

l-NAME and PTIO solution was prepared directly in IMDM.

Bromophenol blue solution was prepared directly before use.

Cell lysates were prepared directly with Laemmli sample buffer (Sigma).

Master batch oils containing 1.0 wt% of different ZrP samples were first prepared by directly mixing each solid powder with mineral oils under mechanical stirring, followed by ultrasonication for about 20 min to obtain homogeneous oil mixtures.

The samples were prepared by directly spotting hot aliquots of a reaction mixture onto a KBr crystal.

Five mixtures of paste, a control mixture and four blended mixtures, were prepared.

"And the result, dutifully prepared, falls directly between the poles".