Sentence examples for mixture of uranyl from inspiring English sources

MVs were postfixed in 2.5% gluteraldehyde, washed and after silver enhancement (BBInternational), embedded in a mixture of uranyl acetate (0.8%) and methyl cellulose (0.13%), and examined in a Jeol JEM 1010 electron microscope.

After rinsing three times with water, the immunostained cryosections were contrasted with a mixture of uranyl acetate and methyl cellulose (25 centipoises, Sigma M-6385) in water, at a final concentration of 1.3% each, for 10 min at RT. Excess liquid was removed and the sections were dried at RT.

Immunogold labelling was carried out on thawed sections with anti-GFP antibodies (1 200) and 10 nm protein A-gold (UMC Utrecht University, Utrecht, The Netherlands) (1 50); a mixture of uranyl acetate and methyl cellulose was used for embedding and negative staining.

After rinsing three times with water, the immunostained cryosections were contrasted with a mixture of uranyl acetate and methyl cellulose (25 centipoises, Sigma M-6385) in water, at a final concentration of 1.3% each, for 10 min. Excess liquid was removed and the sections were dried at RT.

After rinsing three times with water the immunostained cryosections were contrasted with mixture of uranyl acetate and methyl cellulose (25 centipoises, Sigma M-6385) in water, at final concentration of 1.3% each, for 10 min at RT. Excess of the liquid was removed and the sections were dried at RT.

The immunodecorated sections were stained with 2% uranyl acetate and embedded in a 9/1 mixture of methylcellulose/saturated uranyl acetate.

After dehydration, pellets were embedded in Epon, ultrathin cuts were made on a Reichert-Ultracut E and contrasted with a mixture of 2% uranyl acetate/lead citrate.

After dehydration in a series of ethanol washes, pellets were embedded in Epon and ultrathin cuts were made on a Reichert-Jung Ultracut E microtome (Leica Microsystems) and contrasted with a mixture of 2% uranyl acetate/lead citrate.

The grids were incubated with primary antibody against HSP70 (1∶10; anti-rabbit) and 10 nm protein A-gold for 30 min. To try to fix the gold labeling, 2% glutaraldehyde was used for 5 min followed by washing with double distilled water for 10 min. The grids were stained/embedded with a mixture of methylcellulose and uranyl acetate and analyzed by transmission EM.

To further enhance contrast of elastin and collagen, specimens were embedded in LR White resin and counterstained with a mixture of tannic acid and uranyl acetate (Haidar et al., 1992).

Sections were labeled with rabbit anti-GFP (1∶100, Invitrogen) followed by 10 nm gold-coupled Protein A, contrasted with a mixture of 1.9% methyl cellulose/0.3% uranyl acetate, and viewed in a Morgagni electron microscope (FEI Company, Eindhoven, NL).