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Briefly, cells were fixed with 4% paraformaldehyde (PFA) and then incubated with a mixture of TUNNEL buffer (TdT buffer) and FITC-dUTP solution for 60 min.
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As you drive through Norway, the highway system is a mixture of tunnels, roads and ferries that wind through the fjords, so there it was all about the scenery and sitting around campfires together.
It is thought the hole has been caused by a mixture of mining tunnels beneath the ground and heavy rain.
Then the city will mount a multiyear program to fill tunnels under buildings with a cementlike mixture of fly ash and water, starting with tunnels that are within 25 feet of the surface.
It is perhaps fitting that New Jerseyans, always leery of New York's oversize shadow, have greeted the idea of a multibillion-dollar freight rail tunnel with a mixture of skepticism and ennui.
Although the eastern section of the ridge was composed of firm and sturdy rock, the majority of the tunnel passed through a mixture of frail slate that was formed at a steep angle, soapstone, and clay.
Smoke in a section of tunnel was being dealt with by the tunnel's ventilation system.
The cost for each tunnel i depends on the predefined unit cost for tunnel excavation, the area of tunnel cross sections and the tunnel length.
Deepening of the tunnel is likely constrained by engineering principles of tunnel stability in unconsolidated materials.
The clinical relevance of tunnel enlargement is not fully understood.
He was the first to suggest the provision of a mixture of helium and oxygen for workers in caissons and tunnel borings to prevent caisson disease (bends).
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CEO of Professional Science Editing for Scientists @ prosciediting.com