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Then the mixture of target protein and aptamer-AgNPs were injected into the microfluidic channels for colorimetric detection.
The speech signal is a mixture of target and interference with the overall SNR of 0 dB.
where Ra is the sensor resistance in air (base resistance) and Rg is the resistance in a mixture of target gas and air.
We also evaluated the speech enhancement performance for a mixture of target speech and real environmental noise and confirmed that there was an improvement.
Mice were injected i.v. with a mixture of target cells including, 10 106 DNBS-pulsed and 10 106 unpulsed spleen cells, previously labeled with 0.5 µmol/L and 5 µmol/L of carboxy-fluorescein succinimidyl ester (CFSE), respectively.
Six days later, mice were challenged (or not, control) with 25 µg of SYGL peptide in incomplete Freund adjuvant (IFA, 200 µl, i.p .. Three days later, mice received a 1∶1 mixture of target B6×BALB/c splenocytes labeled with 5 µM CFSE (CFSEhi), and control B6 splenocytes labeled with 0.5 µM CFSE (CFSElo) (2×107 total cells in 300 µl PBS / mouse).
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HAEC (60 70% confluence) grown in 12-well plates were transfected with a heterogeneous mixture of target-specific CREB siRNA (50 n m) or scramble sequences of siRNA by using siRNA transfection reagents according to the manufacturers' protocols.
Like GreeneLAMP, VIPR is not designed to deal with complex samples, where a mixture of targets might be present.
While some signal may be gained by reducing the stability of secondary structure in the target molecule, random shearing by its nature creates a mixture of targets that may have substantially different affinities.
It is therefore likely that Group B contains a mixture of targets that could be undergoing miRNA translational repression, transcript destabilisation or indeed alteration due to non-miRNA-mediated processes.
Extract aliquots and cultivation medium supernatants (20 μl each), as well as dilution series of standard mixtures of target metabolites (Table 1), were spiked with internal standards (ribitol, 10 or 1 μg for the extracellular or intracellular analysis, respectively) and lyophilized.
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