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Chase L. Beisel at North Carolina State reported that Crispr could be used to kill one strain of bacteria in a mixture of strains, by targeting a sequence unique to that strain.
Consequently, the C. glutamicum species dropped into a mixture of strains from diversified resources.
Inoculants consisted of a mixture of strains CNPSo 2083 (=Ab-V5) and CNPSo 2084 (=Ab-V6) of A. brasilense (from the Collection of Diazotrophic and Plant Growth-Promoting Bacteria of Embrapa Soja, WFCC # 1213, WDCM # 1054).
The DNA mixture of strains 91001 and EV76 were used as positive control.
Group Ax contained a mixture of strains belonging to A194 and a second, uncharacterized Cp23S-rDNA clade A genotype called A188 and contained all full-length COI clade A sequences deposited in Genbank.
Sabkhet El Melah showed the most diverse community displaying a mixture of strains affiliated into 11 genera.
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Conclusions: The CMV isolates obtained from our patient were complex mixtures of strains.
Two mixtures of strains exhibited greater reduction effects, i.e., 35% and 56%, respectively.
The developed library of characteristics can help in the design of mixtures of strains to obtain a specific melange of product functionalities.
The mixtures of strains tested in laboratory core flooding based MEOR also confirmed their greater MEOR performance, i.e., MEOR levels of 9.1% and 13.2%, respectively, compared to that of any single strain ranging from 7.0% % to 8.7%.
A further advantage of monitoring individual colonies is that when mixtures of strains with different susceptibilities are present the effect on a subpopulation of the colonies can still be readily and rapidly be identified (Figure 4).
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