A host of other surreal elements think toothy teleporters, tiny baby hands, and strange ritualistic dance numbers are all rendered in a mixture of stop-motion, ace make-up design, campy but believable costumes, and other briliant practical effects.
After transcription mixtures had been incubated for 2 h at 37 °C, 2.5 μL of 0.5 M EDTA and 6 μL of 50% glycerol were added for every 30 μL of transcription mixture to stop reactions.
At desired times, 2 μL of chlorination assay mixture was added to 8 μL of a 0.5% TFA/H2O mixture to stop the reaction.
To 15 μL of the solution from the chlorination assay described above was added 85 μL of a 0.1% TFA/H2O mixture to stop the reaction at a specified time, followed by HPLC using a solvent gradient from 20 to 40% B for 5 min and 40 to 65% B for 20 min (solvent A, 0.1% TFA/H2O; solvent B, 0.1% TFA/MeCN).
One µl of 0.5 M EDTA was added to the mixture to stop the reaction.
The cleavage reactions in Figures 1, 3 and 4 were started by the addition of enzyme and then incubated at 30°C for the indicated amount of time before being stopped by the addition of one-fifth volume of stop mixture (2.5% SDS, 200 mM EDTA, 10 mg/ml proteinase K) followed by a further 15 min at 30°C to deproteinize the mixture.
We use approximately 600 words for this task (a mixture of commonly-used stop-words).
The immediate Western response was to find some way, through threats, diplomacy or a mixture of the two, to stop the Russians going farther into eastern Ukraine.
After incubation, 10 μl aliquots from the reaction mixture were added to 90 μl of stop solution (1M NaOH, 1M glycine, pH 10) and the fluorescence was measured at excitation/emission of 370/440 nm (TECAN Infinite M200).
A Manchester Guardian reporter walked with the Lancashire party, noting the event's mixture of fisticuffs and decorous stops for cups of tea, as well as the final procession back to Hayfield, singing the Red Flag behind the local police inspector in a "baby" Austin car.
The music is a glorious mixture of Wagner, minimalism, show-stopping songs, fine melody and jazzy rhythmic complexity, and everyone is enjoying the clever humour of the text ("Theseus is clever / Theseus can box / his arrows are unerring and he works out with rocks").
