HPTLC offers a great variety of stationary phases with unique selectivity for mixture components and their separation simultaneously.
Peptide mixtures were run at pH 2 on columns with a variety of stationary phase selectivity (C8, C18, polar endcapped, polar embedded, ether-linked phenyl and Phenyl-Hexyl) under linear gradient conditions with acetonitrile or methanol as organic modifier.
The small variability in the results obtained in various experiments is attributed to the differences in the rate of cell division within the filaments aggregates, which resulted in a mixture of dividing and stationary phase cell populations.
It has been observed that, generally, the Comon algorithm successfully achieves to separate instantaneous mixtures of non-stationary signals, despite it has been designed for stationary environments.
The mixture was added to 1 – 2 ml of stationary phase grown Mycobacterium smegmatis mc 2 155, in a tube to which 9 ml of molten LB agar (1.5%) was added.
In addition, mixtures of SCMSV peptide standards to assess overall capabilities of stationary phases to resolve complex peptide mixtures underlined the useful complementarity of combinations of different columns and elution conditions to maximize flexibility in peptide applications.
Gradient conditions were different in each case, adjusted to the dimensions and demands of each stationary phase (mixture of acetonitrile and water acidified by HCOOH at pH 3.2).
In order to examine the merit of the two-step sequential method, in which two perovskite precursors are sequentially sprayed, perovskite films were also fabricated by one-step co-spray deposition of a mixture of the two precursor solutions on stationary (run 9m) and vibrating substrates (run 10m).
Gilar et al. separated and analyzed a mixture of approximately 200 peptides using different stationary phases, including SEC, HILIC, RP at pH 2.6 and pH 10 and SCX.
