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Briefly, a reverse transcription is performed with a mixture of specific primers designed against all known miRNA precursors.
To do this, we had observers search for a mixture of specific and categorical target types.
An improved renoprotective cocktail referred to as I-GPM (a mixture of specific renoprotective growth factors, porphyrins, and mitochondria-protecting amino acids) was administered -24 hours, 0 hours, and +24 hours after surgery.
Cells previously exposed to AgNPs or AuNPs were incubated with PBS 4% FBS containing a mixture of specific fluorochrome-labelled antibodies used at appropriate dilutions for 20 min at 4 °C, according to the procedure specified by Ayres-Martins et al. [11].
As expected, when tested concurrently with a mixture of specific peptides derived from ESAT-6 and CFP-10 of M. tuberculosis, samples from patients with active and inactive TB did react in the ELISPOT assay, whereas samples from all healthy individuals, did not (Fig. 2 A and B).
Decachlorobiphenyl (DCBP - IUPAC number 209) was used as an internal standard, added to each sample extract prior to analysis, and included in the calibration standard, a mixture of specific compounds (Aroclor 1260, HCB and pp'- and op'-DDT, DDD and DDE).
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To quantify the mutant cells in the original sample, we compared fluorescence obtained from the experimental sample against a standard curve generated by the fluorescence detection from known mixtures of specific cell lines generated to express the gene of interest with or without the mutation of interest (see Supplementary Table 4 for conditions of cloning these plasmids; Fig. 3e).
In the 2008 2010 cross-sectional study, specific IgE for mixtures of specific indoor and outdoor allergens were measured in a subsample of 180 children.
However, recently it has been shown that linear ion traps can be rather more appropriate in certain analytes such as mixtures of specific peptides in biological specimens [ 6].
Consisting of mixtures of specific, evolutionarily conserved compounds with discrete pharmacologies, snake venoms provide an unusual opportunity to quantitatively study genomic consequences of ecological interactions, particularly how natural selection acts on rapidly co-evolving gene complexes.
Observers could be asked to look for any of three specific target types, three categorical target types, six specific, six categorical, or a mixture of three specific and three categorical target types.
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