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Briefly, Protein G columns were equilibrated with binding buffer (Immunopure IgG, Pierce , USA after which a 1 1 mixture of sera and binding buffer was allowed to flow through under gravity, washed and eluted with elution buffer (Immunopure IgG, Pierce , USA.
The only difference was the starting sample used which, in this case, was a mixture of sera derived from two patients.
The mixture of sera by a 2-fold dilution and the standard virus was added to MDCK cells in the microplate.
Particles were purified and detected by Western blotting using either the anti-E 4E11 monoclonal antibody or a mixture of sera from four patients who were infected by all dengue serotype (Figure 8A B).
E proteins of all DVs were detected using the mixture of sera (Figure 8B) with a signal of similar intensity, which suggested that RSPs of four serotypes could be generated to comparable levels.
A positive control, consisting of a mixture of sera from DBA/1 mice immunized with the protein in question, was used on all plates assayed.
Similar(52)
Mixtures of sera #32 and #3 gave positive results in the bispecific antibody assay, which became weaker with increasing dilutions of #3 (Fig. 4A).
The relative rate of serum ACE2 enzyme activity was then compared with a reference mixture composed of sera from healthy subjects.
Using the Human Epigenome Microarray Platform HEMPP) [ 36], we compared these SLE sera to each other as well as to control sera from nine healthy adults and to a positive control comprising a mixture of autoimmune sera with defined reactivity.
Anti-rZH501-M847-G serum demonstrating a PRNT80 titer of 1∶640 to ZH501 was a mixture of the sera, each collected at days 6, 7 and 8 p.i. from eight rZH501-M847-G-infected mice.
Similarly, anti-rZH501-M847-A serum demonstrating a PRNT80 titer of 1∶80 to ZH501 was a mixture of the sera collected at 6 days p.i. from four rZH501-M847-A-infected mice.
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