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Then a mixture of reaction buffer, RNaseOUT and reverse transcription enzyme was added.
First attempts to prepare cycloSal-PMEAs 209, directly from the diethyl ester of PMEA 48, without protection of the exocyclic amino group, led to a complex mixture of reaction products.
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It is an odd mixture of reactions.
The mixture of reactions to Mr. Clinton affirms the complexity of analyzing his leadership.
It causes a mixture of reactions – surprise, charm, excitement among the men, a fair amount of embarrassment, jealousy.
So, it was a mixture of reactions.
The recent Supreme Court decision on the Roundup Ready Alfalfa case (PDF) has drawn a mixture of reactions from the mainstream media, and from the Blogosphere.
There was a mixture of reactions to recommended practices for dealing with asphyxia.
Following the mixture of each reaction, qRT-PCR was performed with a thermal cycler Rotor-Gene QQiagengen, Venlo, Netherlands).
The mixture of each reaction contains 3 μL total RNA, 20 μL Multiplex RT-PCR Buffer, 2 μL Multiplex Enzyme Mix in a total volume of 25 μL.
Two μl of the reaction mixture of the first reaction containing the 278 bp amplificate were used as template in the second PCR reaction in a total volume of 20 μl.
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