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Therefore, the current method allows simultaneous detection of different types of receptors using a mixture of peptide ligands.
We found that priming with the polyepitope construct and boosting with the mixture of peptide in A2-transgenic mice resulted in: (1) CTL responses not only against the peptide-sensitized T2 and SW480 cell lines but also the non-sensitized reconstructed B16 cell line; (2) expression of HLA-A*0201/H-2Kb chimeric gene and polyepitopes by B16 led to its rejection by immunized A2-transgenic mice.
Mass spectra of whole proteins were acquired on linear mode using a matrix solution of α-cyano-4-hydroxycinnamic acid in 0.2% trifluoroacetic acid in acetonitrile-H2O (50%, v/v), and were externally calibrated using a mixture of peptide standards (Applied Biosystems).
In-gel digestion was performed on each gel slice and the mixture of peptide digests was extracted from gel.
Moreover, the labeling procedure is straightforward: the mixture of peptide and Cu only needs to be incubated at 37 °C for less than 60 min.
Calibration was conducted using a mixture of peptide standards (MSCal1; Sigma-Aldrich), and only masses above a signal to noise ratio of 5 - 10 were recorded.
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It consists of a mixture of peptides with high and low cystine content.
The 70% DMAEMA brush was used to demonstrate an efficient approach to alleviate the ion suppression effect in MALDI analysis of complex mixtures by effectively fractionating a binary mixture of peptides prior to MALDI-MS analysis.
As it consists largely of a complex mixture of peptides, it has been subjected to systematic peptidomic study but there has been little focus on molecular cloning of peptide-encoding cDNAs and by deduction, the biosynthetic precursors that they encode.
While bulk peptides obtained by mild acid elution from LCL contained multiple T-cell epitopes, this complex mixture of peptides was poorly immunogenic, even when presented by mature dendritic cells (DC).
By using a mixture of peptides of this domain or a mixture of such hybrid peptides the type-specificity of the neutralizing antibody response to this determinant can perhaps be overcome.
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