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Forty New Zealand white rabbits were sensitized twice with mixture of OVA and aluminium hydroxide on days 1 and 14.
It has previously been demonstrated that rats fed ovalbumin (OVA) and subsequently immunized subcutaneously with a mixture of OVA and human serum albumin have significantly lower IgE antibody and lower delayed-type hypersensitivity reactions to human serum albumin than controls [ 1].
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A similar suppressive effect was not observed in mice that received empty OMLs or a mixture of soluble OVA and empty OMLs, suggesting that effective antigen delivery by antigen-carrying OMLs is important in induction of systemic immune responses.
Mice were sensitized using a mixture of SWF and OVA (serum OVA-specific IgE was detected with a highest titer as 1 64).
One week after the last day on the OVA diet, mice were anesthetized with isofluorane and immunized subcutaneously at the base of the tail with a freshly prepared emulsion (100 μl) containing acetic acid, BCII, OVA or a mixture of BCII and OVA (100 μg of each antigen), and an equal volume of Freund's complete adjuvant.
Indeed, the OVA-tolerized mice that were subsequently coimmunized with a mixture of BCII and OVA had reduced arthritis and a decreased number of splenic IgG anti-BCII antibody-producing cells and serum IgG2a anti-BCII antibody activity.
In the current study, we investigated whether oral immunization with a mixture of soluble and particulate OVA plus CpG ODN and polyphosphazene adjuvants could promote oral immunity.
Briefly, to a mixture of 90 mg of liposomes and 6 mg of OVA in 2.5 ml phosphate buffer (pH 7.2), 0.5 ml of 2.5% glutaraldehyde solution was added in dropwise fashion.
C57BL/6 mice were treated weekly with OVA alone (10 μg/mouse) or with a mixture of C60-P at varying dose rates and OVA by intraperitoneal injection.
DBA/1 mice were fed ovalbumin (OVA -containing pellets ad libitum fOVA -containingubsequently coimmunized twice, with a mixture of bovine collagen typelletsBCII) add OVA in Freund's complibitumjuvant.
OVA conjugated TMC (OVA TMC) significantly increased uptake of OVA by Raw 264.7 cells, which was 2.38 times higher than that of OVA/TMC physical mixture (OVA + TMC) at 4 h.
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