Sentence examples for mixture of mutants from inspiring English sources

In addition, all 27 oligonucleotide tags were amplified specifically when genomic DNA from a mixture of mutants was used as the template (Fig.  2B).

This mixture of mutants, also called a mutant cloud or quasispecies, does not necessarily have to contain the fastest-replicating individual sequence that exists in the fitness landscape.

This was indicative of an overall transfection efficiency of ∼10−4 and suggested that roughly 2,500 independent recombination events occurred in a transfection, enough to generate a complex mixture of mutants.

Thus upon mutagenesis and transformation of E. coli, a mixture of mutants was obtained where each bacterial colony on a plate expressed TetA(B) that had been mutated at a single amino acid position, but which contained either a Val, Ala or Gly residue.

The proposed method exhibits high sensitivity with a detection limit of 1.4 pM and can accurately discriminate as low as 1% variant frequency from the mixture of mutant target and wild-type target.

The mixed infection experiments employed a ∼1∶1 mixture of mutant and wild type strain.

It is possible that the PCR reaction contains a mixture of mutant and non-mutant MDM2 fragments.

A portion of each homogenate was pooled to obtain a mixture of mutant and WT mice cerebellar proteins, respectively.

As the Keio aroD mutant was reported to be a mixture of mutant and other strains [ 36], a colony was isolated and confirmed to be mutant by PCR.

Genomic PCR: perform PCR with the DNA samples, one WT sample as negative control and one mixture of mutant and WT alleles as positive control if available.

(D ) Single-particle fusion kinetics (left) and fusion yield (right) for WNV VLPs containing a 1 2 mixture of mutant (W101A) and wild-type E; compare with Figure 7D.