The experiments are carried out with a mixture of mm-size granules of copper and PVC, originating from mechanically processed electric wire wastes.
Cells were also treated with maleimide-biotin after a preincubation with 10 mM MTSES, a mixture of 10 mM MTSES and 1 mM estrone-3-sulfate, or a mixture of 10 mM MTSES and 0.75 mM estradiol-17β-glucuronide. Maleimide biotinylation of L545C was completely prevented by preincubation with MTSES, indicating that L545C indeed reacted with MTSES.
M. acetivorans strains were grown in 10 ml TMA and then transferred to 125 mM MeOH, 120 mM acetate, or a mixture of 40 mM acetate and 125 mM MeOH.
In the first, both cells of the plate contained the equivalent of N-free half strength MS medium and N was administered as a mixture of 3 mM NO3− +1.5 mM glutamine.
The cleaned electrodes were incubated in a mixture of 5 mM MUA/5 mM MU (1 3, v/v) for 48 h.
HS mineral salt medium was supplemented with the carbon source 50 mM trimethylamine (TMA), 125 mM MeOH, 120 mM sodium acetate, or a mixture of 40 mM sodium acetate and 125 mM MeOH (21).
In sessions 5 and 6, each rat received infusions of 30 mM amphetamine and the mixture of 30 mM amphetamine and 3 mM sulpiride.
In sessions 2 3, each rat received injections of amphetamine (30 mM) alone and the mixture of 30 mM amphetamine and 1 mM SCH23390.
In one set of experiments, a mixture of 20 mM potassium phosphate, 1.4 mM NAD+, 1.8 mM ATP, and 5 mM MgCl2 was also added.
A mixture of 5.5 mM polyP, 150 mM freshly dissolved EDAC, and 280 mM MU in a reaction volume of 1 mL was incubated for 1 h at 65 °C with agitation because the concentrations of MU used exceeded solubility limits.
A mixture of 5.9 mM polyP, 150 mM freshly dissolved EDAC, and 200 to 525 mM NOL was incubated for 1 h at 65 °C.
