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Although TB positive sera generally gave better binding to the antigenic MAs than TB negative sera, no single antigenic MA was significantly better able to differentiate between TB positive and TB negative sera than the natural mixture of MAs could.
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The main issue of this manuscript is the exploration of the solubility of methacrylic acid (MA), and the oligomers of poly ethylene glycol) methyl ether (MPEG), poly ethylene glycol) methacrylate (PEGMA), and a mixture of MA and PEGMA.
However, none of the individual synthetic mycolic acids significantly and reproducibly distinguished the pooled serum of TB positive patients from that of TB negative patients better than the natural mixture of MA.
A biosensor approach, the MARTI-test (Mycolic acids Antibody Real- Time Inhibition), using free natural mixtures of MAs in liposomes as antigens in a competitive binding assay showed improved accuracy (Lemmer et al., 2009; Thanyani et al., 2008).
A more sensitive and specific diagnostic assay could possibly be developed by making use of specific stereoisomers of single chain lengths of synthetic MA subclasses instead of using natural mixtures of MA.
During the compounding process, the compatibilizers further react with PA6 in situ to afford the mixture of PP-g-MA-co-POP-PA6, PP-g-MA-co-POP-co-PA6 and PP-g-MA-co-PA6 copolymers, which further improves the compatibilizing effect.
Significantly, to enhance solar light harvesting capability, a mixture of methylammonium (CH3NH3+, MA) and formamidinium (HNCHNH3+, FA) cations were explored to synthesize superior performance perovskite materials.
For a typical experiment of InP NQDs('standard reaction'; Figure 1), 0.04 mmol of In OAc 3 was added to a mixture of 0.12 mmol MA and 4 ml ODE in a 50-ml three-necked flask.
The use of the complex mixtures of natural MA is complicated by an apparent antibody cross-reactivity with cholesterol.
For a typical experiment, 0.04 mmol of In OAc 3 was added to a mixture of 0.12 mmol of MA and 4 ml of ODE in a 50-ml three-necked flask.
Saturation kinetics of DA uptake was determined using a mixture of [H]DA (PerkinElmer Life Sciences, Waltham, MA) and unlabeled DA (Sigma Aldrich) diluting to final DA concentrations of 0.05 μM–10 μM.
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