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Furthermore, a difference in T2-weighted signal reduction was observed between the 100% labeled NSCs and non-labeled NSCs, as well as in an equal mixture of labeled and non-labeled NSCs.
Selective labeling of IgFLNa19 21(1 C-L2271,N-A2272) was produced using a mixture of labeled (1-C-leucine and N-alanine) and non-labeled amino acids (0.1 g for each per liter M9) (Peterson et al, 2001).
WT V154C gave a mixture of labeled and unlabeled species, and suppression of BIM reduced the IASD-labeled species, although this effect was only evident following ABT-737 treatment.
Methods have been proposed for learning based on a kernel function from a mixture of labeled and unlabeled data [24], alleviating the problem of small-sized training data.
The DNA is then cut with a restriction enzyme and the sizes of the resulting mixture of labeled terminal restriction fragments (TRF) are determined through fragment analysis.
The simultaneous processing of a mixture of labeled and unlabeled samples allows a sensitive and accurate comparative analysis between the respective proteomes.
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All of the following SIL-assisted approaches involve analysis of mixtures of labeled and non-labeled samples and are based on enrichment of the respective mass spectra with the isotopic pattern of the SIL analogue(s).
Even mixtures of labeled compounds and structurally simple precursors such as 13CO2 can be used as tracers on the basis of the retrobiosynthetic approach.
The sequences of these probes are designed to interact with selected mixtures of labeled nucleic acids, called targets [8].
The reaction mixture consisting of labeled γP-ATP (9 μCi) and unlabeled ATP (1 mM), 1 10 μg of recombinant PfClpY protein in 20 mM Tris-HCl (pH 8.0) buffer containing 8 mM DTT, 1.0 mM MgCl2 and 10 mM KCl was incubated at 37 °C for 2 h followed by thin-layer chromatography.
Primers were purchased from MWG Biotech, Inc. (High Point, NC, USA), suspended to a concentration of 100 uM in 10 mM Tris, 1 mM EDTA pH 7.4 and used at a final concentration of 0.2 mM in a mixture of 3 2 (labeled:unlabeled) for the forward (IRD700-labeled) primers and 4 1 (labeled:unlabeled) for the reverse (IRD800-labeled) primers as per Colbert et al., 2001 [ 52].
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