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We centrifuged the mixture of granules and antibodies over a discontinuous Percoll gradient and isolated the intact azurophilic granules from the appropriate gradient fraction.
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NETs are released from activated neutrophils and comprise a mixture of granule proteins, antimicrobial peptides and nuclear constituents such as chromatin and histones that combine to form extracellular fibers capable of trapping and killing bacteria (Brinkmann et al., 2004).
The inoculum consisted of a mixture of clay granules, infected root pieces, and AM fungi spores of Glomus claroideum, Glomus intraradices, Glomus mossae, and Glomus geosporum (Symbio-m, Lanškroun, Czech Republic).
A standard mixture of hydroxyapatite granules, fibrillar collagen (Spongostan Powder, Johnson & Johnson, Skipton, UK), and the patient's blood was prepared according to the technique recommended by Byrd [ 24].
There was a relationship between the wetting of the powder mixture and forming of granules prepared with high-shear granulator.
To achieve a perfect mixture of the HDPE granules with MWCNT particles, extrusion process with two extruders was utilized.
The experiments are carried out with a mixture of mm-size granules of copper and PVC, originating from mechanically processed electric wire wastes.
For release from granules, 15 μg of a mixture of specific and gelatinase granules, isolated as described above, was incubated with azurosome containing the equivalent of 50 nM NE (as measured by ELISA and semiquantitative immunoblot) in the absence or presence of 1% NP-40 for 1.5 hr at 37°C.
This formation comprises terrace deposits displaying a mixture of poorly sorted sand, granules, pebbles, cobbles and boulders with some sand intercalations.
In order to experimentally analyse multiphase flow patterns in a mixture of water, air and granules in the SBR, optical in situ techniques are applied.
The investigational therapy (Artequin Pediatric) consisted of artesunate 50 mg/day (2.5 5 mg/kg/day) and mefloquine 125 mg/day (6.25 12.5 mg/kg/day) given in equal oral doses simultaneously in a fixed-dose formulation (stickpack with a mixture of artesunate and mefloquine granules) once daily for 3 consecutive days.
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