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Fig. 5. (a) Interior of the impact crater at location A. The material filling the crater is a mixture of fragments of lava, wood and soil.
A mixture of fragments was obtained [24].
Therefore, the LMW fraction can be considered to be primarily CTF, whereas the HMW fraction is a mixture of fragments from both terminal domains.
It can be performed with a mixture of fragments, thus increasing the length of the DNA fragment that can be analysed in a single experiment.
DNA cloning is a powerful simple method for purifying a particular DNA fragment from a complex mixture of fragments and producing large numbers of the fragments of interest.
To reduce the complexity of the mixture of fragments, a series of PCR amplifications were performed to generate subsets of fragments using selective primers.
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If an MS/MS experiment without ion mobility separation is performed, the resulting spectrum will contain a mixture of fragment peaks from the fragmentation of both species.
The algorithms utilize the fact that each read fragment is derived from either one of two chromosomes, though the observed data are a mixture of fragment data from both the chromosomes.
Rather than using restrictive enzyme (RE) as done in conventional Hi-C, which generates predictable and consistent fragment ends, DNase I produces a heterogeneous mixture of fragment ends that greatly improves the efficiency and resolution.
As WAC-MS can be multiplexed by analysing mixtures of fragments (20 100 fragments) in one sample, this approach yields high throughput, where a whole library of e.g. >2000 fragments can be analysed quantitatively within a day.
Additionally, mixtures of fragments derived from OA cartilage have been observed to promote chondrolysis in vitro [ 17].
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