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The mixture of formaldehyde and organic amine was used as the solvent in the present study.
The cadaver had been embalmed with a mixture of formaldehyde, phenol, polyethylene glycol and alcohol, which has been shown not to significantly affect the stiffness of bone [14].
Sodium deoxycholate was easily identified in this mixture with the chosen TLC system (Figure 6). Figure 6 Densitographic presentation for identification of sodium deoxycholate in the mixture of formaldehyde, Triton X-100, Tween 80, sodium deoxycholate.
The arteries are embalmed by simultaneously introducing embalming fluid (a mixture of formaldehyde, other chemicals, and water) into an artery while draining the blood from a nearby vein or from the heart.
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In brief, immediately after removal of the medium, cell monolayers (infected and noninfected) were fixed for 2 h in a mixture of 4% formaldehyde and 2.5% glutaraldehyde in 0.1 M sodium cacodylate buffer, pH 7.2, containing 5 mM CaCl2 at room temperature.
Third instar wing discs were dissected out in cold PBS, fixed for 30 min at room temperature in a 1 1 mixture of 8% formaldehyde: PEM 2× (0.2 M PIPES, 2 mM MgCl2, 2 mM EGTA) and permeabilized in PBS + Triton 0.3% (PBT) at room temperature (3 washes, 20 min each).
The major part was immersed for 24 to 72 h in a mixture of 2% formaldehyde + 2.5% glutaraldehyde + 0.02% sodium azide in 0.05 M Na cacodylate buffer, pH 7.2.
The terminal portion (about 2 mm) of each root of the control and the treated groups were cut and fixed in a mixture of 2% formaldehyde and 2.5% glutaraldehyde in 0.2 M phosphate buffer (pH 7.2) for 2 h and then thoroughly washed with the same buffer three times.
At the end of this experimentation period the cultures were fixed for 30 minutes in a mixture of 2% formaldehyde and 0.2% picric acid in 0.1 M phosphate-buffer, pH 7.2, followed by rinsing twice in Tyrode's solution containing 10% sucrose, and frozen until processed for immunocytochemistry.
Hannouche et al. (2007) reported that fixation using a mixture of 2% formaldehyde and 0.2% glutaraldehyde for 4 days at 4°C gave the best results for X-gal staining of both cell cultures and bone implanted with mesenchymal stem cells from a vimentin- lacZ knock-in mouse.
One portion of each gut segment was fixed in Stefanini's fixative (a mixture of 2% formaldehyde and 0.2% picric acid in phosphate buffer, pH 7.2) for 22 h at 4°C, and the other portion and the uteri were fixed in 4% paraformaldehyde in 0.1 M phosphate buffer for 22 h at 4°C.
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