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Dependent on the histological type, non-seminomas are composed of a disorganized mixture of differentiated somatic cell types and extraembryonic cells, together with EC cells.
In absence of signals and intercellular communication, development would terminate in a salt-and-pepper mixture of differentiated cell types without any pattern.
In recent years, the mammary gland epithelium has been shown to be a mixture of differentiated cell populations in a hierarchical relationship with their stem and progenitor cells.
In Tsai et al.[ 59], 47 genes were found to be a specific MSC core gene signature when contrasting microarray data from four human perinatal tissues against a mixture of differentiated cells.
We favor the idea that only a fraction of the genes that we identified are affected in cell culture because S2 cells do not represent the complex mixture of differentiated tissues that we sampled in the embryo.
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Human myoblasts were cultured for 72 hours in a 50/50 mixture of differentiation medium plus medium conditioned by either hESCs or differentiated hESCs, or in 100% of differentiation medium, followed by mRNA expression analysis by RT-PCR.
However, EBs generally contain a mixture of cells, differentiated into multiple lineages, highlighting the challenge of achieving a homogenous cell population [36].
These tumours contain both embryonal carcinoma (EC) cells, which represent the stem cells of the cancer, and a heterogeneous mixture of their differentiated derivatives.
The histopathological appearance is distinctive in its mixture of poorly differentiated small cell areas with prominent multilayered (ependymoblastic) rosettes, and areas with focal neuronal differentiation, thus combining features of CNS neuroblastoma and ependymoblastoma [ 3, 5, 11].
However, the deviations of these loci were not significant in most locations (including Kaoping and Hainan) when individuals were sorted according to one of the three mtDNA lineages, suggesting a Wahlund effect, which is the reduction of heterozygosity in a sample due to the mixture of genetically differentiated populations.
Heterozygous CreA12 cells initially formed a mixture of undifferentiated and differentiated cells, but by day 9 had mostly differentiated with few remaining ES cells.
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