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Evaluations are as good as their project design: Getting projects designed right means meeting a mixture of conditions.
The approach based on the construction of cDNA libraries from a mixture of conditions was successfully used in T. reesei by Foreman et al. [ 17].
The approach based on the construction of cDNA libraries from a mixture of conditions was previously used successfully in T. harzianum[ 10, 12].
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The growth of the qns1 strain is then assayed in a 1∶1 mixture of conditioned media and fresh 2x SDC such that the extent of qns1 growth is proportional to the extracellular concentration of NR.
Prior to every experiment cells were seeded at 2 × 10 to 4 × 10cells/ml with a 50/50 mixture of conditioned and fresh medium.
The present approach can be used to understand cell decisions when presented with mixtures of conditions.
The first case is derived from the construction of gene expression data, which includes the selection of microarray samples (a specific condition or a mixture of various conditions) and of GeneChip summarization methods [RMA, GCRMA, MAS5 or PLIER (Affymetrix Technical Note, http://www.affymetrix.com/support/technical/technotes/plier_technote.pdf)].
80% of the 1,210 TSSs were detected in the RNA sample pool corresponding to the mixture of growth conditions and 60% of the TSSs were predicted under at least four conditions.
I found both cameras produced good, if not spectacular photos in a mixture of lighting conditions.
This explicitly falls within the concept of the "component cause": the identification of the specific mixture of necessary conditions and events (i.e. duration and level of exposure to the pathogen and the presence of animal- and herd-specific risk factors) which are both necessary and sufficient to produce I. suis infection and determine the future course of the infection [ 16].
At first, for the optimization of the reaction conditions, a mixture of resorcinol, benzaldehyde and malononitrile was investigated as a model and its behavior was studied under a variety of conditions.
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