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The growth of the qns1 strain is then assayed in a 1∶1 mixture of conditioned media and fresh 2x SDC such that the extent of qns1 growth is proportional to the extracellular concentration of NR.
Prior to every experiment cells were seeded at 2 × 10 to 4 × 10cells/ml with a 50/50 mixture of conditioned and fresh medium.
Approximately 1.25×105 of non-adherent PGC-like cells collected at D25 of induced differentiation were resuspended in 1 ml of a 1∶1 mixture of conditioned medium and fresh differentiation medium.
A second unirradiated set received a 1 1 mixture of conditioned medium from irradiated cultures and fresh medium, while a third unirradiated set received a 1 1 mixture of conditioned medium from unirradiated cultures and fresh medium.
After 24 h, the culture medium was replaced with a mixture of conditioned MCDB153 supplemented with 10% dFCS and FAD medium (1 : 1 volume) and cultures incubated for 24 h.
Adenovirus infection (2.6 × 10 p.f.u./insert in a 12-well plate) was carried out on day 1 after seeding onto collagen type I gels in a mixture of conditioned MCDB153 supplemented with 10% dFCS and FAD (1 : 1 volume) for 24 h.
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Evaluations are as good as their project design: Getting projects designed right means meeting a mixture of conditions.
The approach based on the construction of cDNA libraries from a mixture of conditions was successfully used in T. reesei by Foreman et al. [ 17].
The approach based on the construction of cDNA libraries from a mixture of conditions was previously used successfully in T. harzianum[ 10, 12].
A 1 1 mixture of different conditioned medium to BGJb/FCS/Antibiotic, and a 1 1 mixture of DMEM/FCS with BGJb/FCS/Antibiotic were placed in control plates.
Analysis of mixture fraction conditioned profiles of temperature and scalar dissipation rate along the jet trajectory identified different ignition and flame stabilization scenarios on the windward and leeward sides of the reacting JICF.
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