The substrates were treated with a mixture of concentrated sulfuric acid and concentrated nitric acid before usage to functionalize with carboxylic and hydroxyl groups.
In the first stage, three types of acids are utilized, namely, concentrated HNO3, concentrated HF, and a mixture of concentrated HNO3 and HF.
Rumen fluid was collected before the morning feeding from two rumen-fistulated steers fed an equal weight mixture of 40% concentrate and 60% grass hay twice daily at 0800 and 1800 h.
Because of the difference in solubility, repeated partial precipitation of the mixture of sulfides concentrates the polonium in the more soluble fraction, while the bismuth accumulates in the less soluble portions.
Nylon-6 (N6)/nylon-6,6 (N66) blend membranes were prepared from N6/N66 blend solutions in calcium chloride-methanol mixture of the concentrates in order to investigate the possibility of porosity control.
In the experiments utilizing bone marrow from Ink4bKORbfl/fl mice, Lin− cells were infected with two viral constructs (MIG- and Lenti-X-pTuner-based constructs) simultaneously using a mixture of highly concentrated viral supernatant (>10 infectious unit/ml each).
To prepare CNT CEs by spraying method (SCNT), 0.5 g CNT powder was firstly treated with mixture solution of concentrated HNO3 and H2SO4 (v/v = 1/3) under 100°C for 1 h to obtain separated CNTs [20].
The control group was fed concentrate mixture whereas in experimental group 50% of concentrate mixture was replaced with isonitrogenous leaf meal mixture.
The basal concentrate diet consisted of a mixture of 50% commercial concentrate and 50% wheat bran fed at 4 kg/horse, offered twice daily at 04:00 and 16:00 hours, and oat straw offered ad libitum at 05:00 and 17:00 hours.
The basal concentrate diet consisted of a mixture of 50% commercial concentrates and 50% wheat bran fed at 4 kg/mare and offered twice daily at 04:00 and 16:00 hours, while oat straw was offered ad libitum at 05:00 and 17:00 hours.
