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The crude product was purified by silica gel chromatography with a mixture of chloroform and methanol (95/5, v/v).
In earlier research I had often used a mixture of chloroform and methanol to extract lipids from a variety of biological materials, such as red blood cells, chloroplasts, mitochondria, and even egg yolks the last being a rich source of a phospholipid called lecithin.
The collected samples were mixed in a mixture of chloroform and DMSO (1 1, v/v).
A monocrystal of compound 8j was obtained via recrystallization from a mixture of chloroform and methanol.
Then, the dried nanoparticle samples were weighed and redissolved in a mixture of chloroform and DMSO (1 1, v/v).
Prior to the optimization of the radiolabeling parameters, the distribution ratio, D, of 111In in a mixture of chloroform and HEPES buffer containing tropolone had been determined.
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A mixture of chloroform-ethyl acetate-formic acid (163:63:25) was used as the solvent system after several trials.
After this, a mixture of chloroform-methanol and plasma in a volume ratio of 9 : 1 was added to the tubes.
Plates with phloroglucinol, pentazocine, hexachlorophene, pyrocatechine, niclosamide, salicylanilide, and thymol were developed with a mixture of chloroform-methanol (9 : 1, v/v).
The stock solution of GM1 (SantaCruz Biotechnology, California,USA), was obtained dissolving the ganglioside in a 4 1 vol. mixture of chloroform-methanol.
The amount of MEL-A attached onto the beads was determined by the previously described anthrone method [ 38] after being extracted with a solvent mixture of chloroform-methanol (2 1).
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