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The use of such a mutated kinase and radiolabeled ATP analogs allows for the specific labeling of direct substrates of the kinase within a mixture of cellular proteins.
It contains a complex mixture of cellular constituents, enzymes and number of high-molecular-weight toxins, including insect toxins and a vertebrate neurotoxin called alpha-latrotoxin, which is active in humans.
A second strategy advocates first enzymatically (e.g., with trypsin) or chemically cleaving ("breaking") a complex mixture of cellular proteins, and then "sorting" the peptides by one or more steps of chromatography.
This indicates that the basal and the hyperrepressed level of csgB reflects a mixture of cellular phenotypes where the fraction of curli-on cells is significantly lower as compared to the wild type, or it becomes undetectable.
In this study, CAL 27-TF16 cells (Fig. 1D) demonstrated a more heterogeneous mixture of cellular morphologies in the presence of PAC (Fig. 1I) than was observed for non-transfected CAL 27.
It has been demonstrated that PCa may be an assemblage of distinct cell types that collectively construct a succession context referred to as tumor microenvironment, which is a complex mixture of cellular and noncellular factors, and plays an important role in tumor development and tumor cell dissemination [ 16].
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Following this, ePTFE tubings were coated with a defined mixture of extra cellular matrices (henceforth referred to as "OptiMat"; see methods for details) and changes in hydrophobicity were assessed using water contact angle (Figure 2A).
Driven by the premise that peripheral blood leukocytes (PBLs) are major effectors in response to endotoxin and that PBLs represent a composite mixture of several cellular subpopulations we opted to simulate the stochastic interactions particularly in the macrophages and T helper cells.
The ribonuclease protection assay (RPA) is a sensitive method used for the detection of low-abundant mRNA in a complex mixture of total cellular RNA (18).
The test wells are coated with a mixture of viral and cellular proteins from virus-infected cells whereas control wells are coated with material from non-infected cells of identical type.
TCLs contain a crude mixture of all kinds of cellular components, including fragments of the destroyed cellular membrane, intracellular organelles, and cellular RNA and DNA.
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