The resulting plasmid should be a mixture of candidate cDNA-containing plasmids.
Two two-compound mixtures of candidate structures for the Phytophthora α1 mating hormone have been synthesized.
To verify the empirical mixture rule for TSI, the TSI values of several binary mixtures of candidate surrogate components were measured.
With a mixture of multiple candidate biotin derivatives, the (expected) total molar quantity of candidate biotin derivatives in competitive binding systems was over 4.6 μM, unless stated otherwise.
With any mixture of multiple candidate ligands, each competitive binding system in 4.0 mL contained 200 μL SMPP and a PMFS at 40 μL.
In the case where the model that generated the data is in the set of competing models, it is analogous to the mutual information between a new measurement (or sample) coming from a mixture of the candidate models and a model classifier (see Additional file 1: section S3).
This sum of SMPP minima must have large errors, as it was just comparable to that estimated by the same approach for a mixture of a single candidate biotin derivative (Table 1).
With any mixture of a single candidate ligand, each competitive binding system in 2.0 mL contained 50 μL SMPP (washed with the buffer) and a diluted PMFS at 40 μL.
In practice, a mixture of a single candidate ligand or a racemic mixture of a few pairs of enantiomers can be prepared via PCS without purification as a sample for screening by this new method, as long as the overall cost and efficiency are favorable.
A mixture of a purified candidate biotin derivative and its reference ligand (BMPL or BPDEDA) at equal molar quantities was added to Tris-HCl buffer in 2.0 mL containing 400 μL SMPP for the total binding capacity of over 1.6 nmol, and each biotin derivative of interest had the final level of 0.2 μM.
