Sentence examples for mixture of buffer from inspiring English sources

The mobile phase was a mixture of buffer and acetonitrile containing β-cyclodextrin.

For elution a 1∶1 mixture of buffer A and buffer B (same as A but with 0.5 M imidazole) was used.

Prepared samples were centrifuged for 5 min at 3000 rounds per min and the mixture of buffer and serum was poured off.

In this study, a one-step and simple assay for EV71 virus without requirement of thermal cycling can be completed within 60 min in a single tube containing the mixture of buffer, primers, reverse transcriptase and Bst DNA polymerase.

After that, basal perfusion was continued (HP-Control, n = 6, protocol 3A) or switched to the 1 1 mixture of buffer and the respective test solution (HP-NaCl, HP-Ri-Ac, HP-6% HES, HP-10% HES and HP-Gel, n = 6 each, protocol 3B).

In the study groups (PMN-NaCl, PMN-Ri-Ac, PMN-6% HES, PMN-10% HES and PMN-Gel, n = 6 each, protocol 2B), the perfusion mode switched to a 1 1 mixture of buffer and the respective test solution after the first equilibration interval.

A slab gel (or column) can be filled with a complex mixture of buffers (known as ampholytes) that, under the influence of an applied field, migrate to the position of their respective isoelectric points and then remain fixed.

They were next packed into 125 mm × 4.6 mm I.D. stainless-steel columns with the use of home-made apparatus equipped with Haskel pump (Burbank, CA, USA) under constant pressure.> -wrap-foot> aColumn dimensions, 125 × 4.6 mm; silica particle size, 5 μm; pore diameter, 100 Å Elution was carried out with mixtures of buffer and methanol or acetonitrile.

Briefly, cells were fixed with 4% paraformaldehyde (PFA) and then incubated with a mixture of TUNNEL buffer (TdT buffer) and FITC-dUTP solution for 60 min.

For phosphatase treatment, beads were washed twice with wash buffer 1, once with wash buffer 2 (wash buffer 1 lacking phosphatase inhibitors) and once in a 1∶1 mixture of wash buffer 2 and NEB3 buffer (100 mM NaCl, 50 mM Tris-HCl, 10 mM MgCl2, 1 mM DTT, pH 7.9; New England Biolabs).

The PCR mixture consisted of buffer (10x), 2 mM MgCl2, 0.1 mM dNTP, 0.32 mM forward primer, 0.32 mM reverse primer, 0.02 U AmpliTaqGold DNA Polymerase (Life Technologies), 25 ng DNA template, water to a final volume of 12.5 microliters.