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After spiking, the samples were extracted by means of Soxhlet extraction using a mixture of appropriate polar and non-polar solvents for ultratrace-analyses (e.g. hexane/acetone).
After spiking, the samples were extracted with a mixture of appropriate polar and non-polar solvents for ultratrace-analyses (e.g. hexane/acetone).
The following day, sections were rinsed three times in 0.2% gelatin/0.25% Triton X-100 in PBS, followed by a 90 min incubation in a mixture of appropriate secondary antibodies.
Mixture of appropriate standards was used for calibration and Schatz and holo-Schatz peptide analytes were analyzed as described above and calibration was performed using the known protonated molecular ion (MH1).
After 24 h, the mixture of appropriate volume of virus stock solution and cell culture medium was added to cells.
The SNP-IT primer was extended for a single base with DNA polymerase and a mixture of appropriate dideoxynucleotide tagged with either fluorescein isothiocyanate (FITC) or biotin.
Similar(51)
The BGE and SHL composition, as well as instrumental parameters, were previously optimized with mixtures of appropriate standards to provide the highest signal-to-noise (S/N) ratio and best resolution.
Additional information about the primary and secondary antibodies is included in Table 1.> Double and triple immunolabeling were performed on alternate series of sections, which were incubated overnight at RT with cocktails of primary antibodies mixed at optimal dilutions and subsequently detected using mixtures of appropriate secondary antibodies.
A mixture of the appropriate acetophenone 1a-d (200 mmol), dimethylamine hydrochloride (260 mmol) and paraformaldehyde (260 mmol) in absolute ethanol (35 mL) and a catalytic amount of concentrated hydrochloric acid (0.5 mL).
A mixture of the appropriate N- ω-aminoalkyl -phenylpiperazine (9–13) (1.0 mmol) iN- ω-aminoalkyl -phenylpiperazinel) was cooled dowN- ω-aminoalkyl -phenylpiperazinehloride 7 or 8 (1.2 mmol) was added at 0 °C iN- ω-aminoalkyl -phenylpiperazine
A mixture of the appropriate aldehyde (10 mmol), rhodanine (20 mmol), and sodium acetate (20 mmol) in glacial acetic acid (10 ml), was heated under reflux for 48 h.
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