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The 18F-gel mixture is withdrawn into a syringe and injected into the lower part of the mold to avoid air bubbles.
Na-heparin is pumped to the tip of a double lumen catheter and the blood heparin mixture is withdrawn continuously at a mixing ratio of 1 1 at a flow of 4 ml/hour.
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The reaction mixture was withdrawn after an appropriate illumination time and centrifuged at 8,000 rpm to settle the suspended solids.
To investigate the hydrolysis pattern of G-Rc by BG-1 with reaction time, the reaction mixture was withdrawn at regular time intervals during the enzymatic hydrolysis.
Two milliliters of the reaction mixture were withdrawn at regular time intervals, and extracted twice with 2.0 ml of water saturated n-butanol.
An aliquot (0.5 mL) of the reaction mixture was withdrawn and the reaction was quenched by adding 1.5 mL pre-chilled trichloroacetic acid (10%%).
The reaction mixture was withdrawn periodically by using a syringe tube and then centrifuges (REMI, R-8C BL BENCH TOP CENTRIFUGE) to separate the suspended clay particles.
After each given irradiation time, about 4 mL of the mixture was withdrawn and separated by centrifuging to remove the suspended solid catalyst.
Reaction mixture was withdrawn at specific intervals, filtered, and analyzed using a gas chromatograph (N2 carrier gas; injection port temperature, 503 K; column temperature, 353 K; at a heating rate of 3 K; detector temperature, 523 K).
At fixed intervals, 10 μL, the reaction mixture was withdrawn to monitor the course of the reaction by high-performance liquid chromatography (HPLC), which has been described in our previous description (Wang et al. 2014, 2013).
Then, 50 μL reaction mixture was withdrawn and mixed with 0.95 mL sodium borate buffer (200 mmol/L at pH 9.2); to this alkaline solution in 1.0 mL, a total of 50 μL TNBS aqueous solution (0.4%) was added and mixed by vortex.
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CEO of Professional Science Editing for Scientists @ prosciediting.com