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Heat from the mixture is removed within the two regenerators of this tandem system.
The vaporized ethanol-water mixture is removed through a side draw and is then fed into the rectification column without condensation.
Soak the skin in this mixture overnight and rinse several times until the salt mixture is removed.
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The remaining water from this mixture was removed azeotropically using acetonitrile.
In each group 20% of the cement content of the control mixture was removed, resulting in starting mixtures with 200, 240, 280, and 320 kg/m3 cement content.
The air bubbles in the mixture were removed through degassing in a desiccator under a vacuum of 2 kPa for 1 h.
Unreacted 64CuCl2 and biotin-PEG-RGD2 in the reaction mixture were removed by centrifugation using a spin column (molecular weight (mw) cut-off 7 kDa).
After the tubes containing the first mixture were removed from the thermocycler and kept in a freezer at − 4 °C for 3 min, 6.5 µL of the second mixture was added to each tube.
Small aliquot of the reaction mixture was removed every 5 min and centrifuged at 20,000 rpm for 10 min to remove the adsorbent particles and measured the absorbance by UV Vis spectrophotometer.
10 mg of oil sample was mixed with 1 mL of toluene and then 2 μL of the solution mixture was removed diluted with 1 mL of toluene/methanol (1 1 v/v) solution.
The transfection mixture was removed and replaced with complete medium.
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