At last, the mixture was distributed into four tubulated bottles (see Fig. 3) for the experiments.
Infected cells were then diluted in warmed LB to a concentration of 0.3 infected bacteria per 50 µL, and 50 µL of the mixture were distributed in each well of four 96 wells microplates.
For both direct plating and enrichment plates, the MBTA/phage/bacterial mixture was distributed evenly on a plate of 7H10 agar (Difco) supplemented with carbenicillin, cycloheximide, 1 mM CaCl2 and 10% albumin dextrose complex (ADC).
The cell-alginate mixture was distributed into the wells of a 6-multiwell culture plate (Nunc) for further flow cytometric studies.
After immediate mixing and incubation for 20 min at room temperature, 400 μL of the mixture was distributed dropwise into cultured COS7 cells in a 6-well plate.
The mixture was distributed into disposable agarose plug moulds (Amersham Pharmacia Biotech, Uppsala, Sweden) and left to solidify for 15 min at 4°C.
Reaction mixtures (10 μL) were prepared for each panel, as shown in Table 2, and approximately 4.6 μL of this reaction mixture was distributed throughout the partitions within each panel using the Fluidigm IPC Controller (Fluidigm, South San Francisco, CA, USA).
The current of blocks for the substrate/toxin mixture was distributed broadly with two populations located around 8.2 ± 0.9 pA (lower) and 26.8 ± 1.1 pA (higher), i.e., relative conductance of 13.1% and 42.9%, respectively.
A positive control mixture was distributed to a row of wells on each plate by mixing 50 μl of cell suspension with 2000 cells per well in 1× MEF media with high concentration 5-aza-2′-dC (10.0 μM).
In situations where the prevalence of PV is distributed as a mixture of beta distributions, the results were basically the same (Additional file 2: Tables S6 S10).
