Sentence examples for mixture in reaction from inspiring English sources

As a negative control, the step using the TUNEL reaction mixture was omitted, and a nucleotide mixture in reaction buffer was used instead.

After rinsing in PBS, the sections were incubated with terminal deoxynucleotidyl transferase plus nucleotide mixture in reaction buffer for 60 min at 37 °C (In situ Cell Death Detection Kit; Roche Applied Science, Indianapolis, IN, USA) as described previously.

After rinsing in PBS, the sections were incubated with terminal deoxynucleotidyl transferase plus nucleotide mixture in reaction buffer for 60 min at 37 °C (In situ Cell Death Detection kit, Roche Applied Science, Indianapolis, IN, USA) as previously described [ 3].

TUNEL reaction mixture (50 μl enzyme solution (TdT from calf thymus in storage buffer) added to 450 μl labeled solution (nucleotide mixture in reaction buffer) and mixed well to equilibrate components) was prepared immediately before use, placed on slides (50 μl/slide), and incubated for 60 minutes at 37°C.

Microwave irradiation of 200 W was applied for 12 23 min to a reaction mixture in a sealed reaction vessel.

To minimize heteroduplex formation, five-cycle reconditioning PCR was conducted using 5 μL of amplification mixtures in fresh reaction mixture, as previously described [ 17].

Previous research [ 28] pointed out that tyrosinase activity is directly related to the concentration of cells or mycelia in the reaction mixture in slightly acidic to neutral reaction conditions.

To minimize hetero duplex formation, five-cycle reconditioning PCR was conducted using 5 μL of amplification mixture in a fresh reaction mixture as previously described [ 19].

Concentration of the levan in reaction mixture is 0.5%.

Final concentration of each substrate in reaction mixture is 10 mM except for levan.

Stirring flow applied in reaction mixture governs the formation of different SBA-15 morphologies [32, 39].