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The internal mixture effect is also approximated using the volume-weighted mixing assumption for multiple different components.
With the formula in Table 1, the expected mixture effect is which indeed resolves to zero.
Table 2 suggests similar behavior for null direct effects when the cell mixture effect is non-null (simulation # 3).
When the mixture effect is null, and estimate with about the same precision, as one would anticipate.
Overall, the analysis suggests that much of the effect of SGA on DNA methylation may be explained by cell mixture, but that the mixture effect is substantially smaller than that occurring for DNA methylation measured in blood.
Here, synergisms are pronounced when the mixture effect is greater than that of the most potent component (for recent examples, see Bouslimi et al. 2008; Kocaman and Topaktas 2010).
Similar(52)
The mixture effect was stronger than the effects caused by the toxins applied singly.
These detected chemicals were mixed in the ratios of concentrations detected and their mixture effect was predicted by concentration addition.
A significant mixture effect was recorded at a dose below the response thresholds for each component.
Most of the remaining samples, when tested as undiluted samples, enhanced the effect of 10 pM E2 and the results from the unspiked extracts indicated that the mixture effect was additive.
Therefore, the idea that default uncertainty factors are overly conservative worst-case scenarios which can account both for the lack of statistical power in animal experiments and protect against potential mixture effects is ill-founded.
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