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At first, experiments were performed using mixture design with pH factor as the process variable.

Optimization step was performed employing a mixture design with pseudocomponents using HNO3, H2O2 and H2O.

The main feature of this article is the construction of simplex-centroid mixture design with the real mixture blends, though with less efficiency.

For this purpose, response surface methodology based in an enlarged simplex centroid mixture design with constrain (∑salts = 100 g/L) was used.

Kenaf seed oil-in-water nanoemulsions were optimised using simplex centroid mixture design with three components (sodium caseinate, gum Arabic and Tween 20).

A set of calibration and prediction samples were created according to a quaternary mixture design with five levels of compaction at each concentration design point.

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Similar(41)

Therefore, compromising on the loss in D-optimality of simplex-centroid design, mixture designs with full mixture blends and with the stable G-optimality can be constructed.

Simultaneous DTA-TG analyses associated with infrared spectroscopy were performed according to simplex centroid mixture designs with three components.

Figure  4 shows the effect of the fed sugar mixtures on the specific protein production rate measured in fed-flask protocol for both mixture designs with either lactose inducer (left) or cellobiose inducer (right).

The process optimization was based on a simplex centroid design (mixture design), coupled with the statistical and graphical analysis of the results.

A crossed design, mixture design combined with process factor (fermentation time), was performed to find the optimal medium formulation and process time.

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