Sentence examples for mixture described above from inspiring English sources

The specificity of the PCR product was confirmed by sequencing with BigDyeTM terminator chemistry using the genetic analyser ABI Prism 310 (Applied Biosystems), β-2-microglobulin (as control for sample integrity) was amplified in the amplification mixture described above and using the following primers: 5'-TCTGGGTTTCATCCATCCGA-3' and 5'-CCCCAAATTCTAAGCAGAGTATGTAA-3'.

Five hundred μL of lysed cells (1 50) were added to 2.45 mL of the mixture described above; then 50 μL of xanthine oxidase, at a final concentration of 2.8 U/L, were added and incubated in a water bath at 27°C for 15 min. The reaction was stopped with 1 mL of 0.8 mM cupric chloride and the optical density was read at 560 nm.

The ligation mixture described above was electroporated into ElectroMAX DH5α-E competent cells (Invitrogen) and the transformed cells plated on 10 cm LB agar plates containing 50 ug/ml carbenicillin.

The N-AMF treatment was not analyzed in experiment 1 but the soil was used as a non-mycorrhizal control in experiment 2. Each rectangular mesocosm (45 cm long×30 cm wide×20 cm high) with a volume of 27 L was filled with 16 kg of the sterilized loam-sand mixture described above.

Because the primer pairs Pq3469, Pq3423 and Pq3313 are suitable for multiplex PCR, they were added to one tube with 1/3 concentrates of the mixture described above.

For the second round of PCR, 2 μL of the PCR product from the first round was added to the 23-μL reaction mixture described above.

Data analysis After software baseline subtraction, the ratio of peak heights for derivatized natural AA-AMP and d15-AA-AMP was determined for the assay mixtures described above.

Cell lines seeded in 24 well plates in quadruplicates at 1×104 cells/well were incubated with Ad5 gl) in the mixtures described above for 30min.

A459 cells were seeded in 12 well plates in quadruplicates at 15×104 cells/well were incubated with virus in mixtures described above for 30 min. Infection media was replaced with fresh growth media containing 10% FCS and cell monolayers were incubated for 2h, 6h, 18h, 30h, 42h, 54 or 66h.

Samples from reaction mixtures described above were applied to a holey carbon film 400 mesh copper grid (Quantifoil Micro Tools GmbH, Jena, Germany) and manually plunge-frozen in liquid-nitrogen-cooled liquified ethane.

To test for the elicitation of human BLyS-specific antibodies following immunization of mice with the BLyS-containing recombinant proteins and mixtures described above, ELISA assays were performed as described above, except that the microtiter plates were coated with recombinant BLyS (2 μg/ml, 100 μl/well).