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A gas mixture containing either 17.2 ppm H2S or 3000 ppb SO2 in balance of N2 was fed into the fibre lumen and was in countercurrent contact with a 10% NaOH solution fed in the shell side of the modules.
Phosphorylation was initiated by the addition of 0.1 volumes of a 10× reaction mixture containing either 2.5 µM [γ-32P]ATP (111TBq/millimole) (Perkin Elmer Life Sciences) and 247.5 µM unlabelled ATP, or 247.5 µM unlabelled ATP alone.
This parent ion was observed in a mixture containing either BvaB1-His or BvaB2-His but not in a blank reaction without enzyme.
The optimised multiplex PCR assay was carried out in 25 µL reaction mixture containing either 2 µL of bacterial lysate, 2 µL of genomic DNA (variable DNA concentrations per reaction mixture) isolated from plant extracts or 100 ng of genomic DNA isolated from bacterial cultures.
Reaction mixture containing either 20 µg of AUF1 transfectants or serum-starved Hela cell extracts, [P]-labelled DNMT1 3'-UTR or CT RNA probe (100 000 cpm/reaction) and 10 µg of tRNA (as non specific inhibitor) were incubated for 1h on ice in the following incubation buffer 10 mM Hepes, pH 7.6; 3 mM MgCl2; 40 mM KCl; 5% glycerol and 1 mM DTT.
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For measurement of responsivity to adenosyl compounds the assay mixture contained either 0.5 mM AdoMet or AdoHcy.
UHPC mixtures containing either dolomite or basalt, and four fiber volume fractions up to two volume percent were investigated.
Intermetallics-reinforced Al2O3 composites have been fabricated by reaction sintering compacts of intensively milled powder mixtures containing either metal oxides and Al or elemental metals, Al and Al2O3.
Mortar mixtures containing either Portland cement or cement blended with slag were specifically designed to halt the hydration at predefined stages without altering the microstructure or promoting further hydration during drying (equivalent systems).
Briefly, binding was optimal for lipid mixtures with an overall negative charge of 50% [24] achieved by 1∶1 mixtures containing either DOPG or PBPS as the negative component, and EYPC as the zwitterionic component.
The triplex-associated fluorescent emission intensities observed from reaction mixtures containing either of the two perfectly matched triplexes were more similar when 2 ng, instead of 1 ng, of hgDNA was present in the 80 µl bulk solution reaction mixtures (Supplemental Tables S7 and S8).
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