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Exact(34)
The reaction mixture contained the suitably-diluted enzyme solution and a 5 mM substrate (neutralized with 1.0 N NaOH) in 0.1 M sodium phosphate buffer (pH 6.0) in a final volume of 1.0 ml.
PCR reaction mixture contained the SYBR PCR master mix (Applied Biosystems), cDNA, and the primers.
The mixture contained the resource that the particular morph type had been reared on during the selection process, and a newly introduced resource.
The incubation mixture contained the appropriate resorufin substrate (5 µM) and 80 µM dicumarol diluted in EBSS (Invitrogen) [29], [30], [36].
In addition to the MEM-protected trans- and cis-cyclopropane products, the crude reaction mixture contained the desired final product, the deprotected trans-cyclopropane, (1S,2S -ethyl 2-(4-hydroxy-3-methoxyphenyl) cyclo-propanecarboxylate (EMDF), which was isolated by column chromatography (90% hexanes/ethyl acetate → 1S,2S -ethylcetate) (99.2- 4-hydroxy-3-methoxyphenyl yield).
Each reaction mixture contained the indicated amount of compound.
Similar(26)
Vaam, which stands for vespa amino acid mixture, contains the same amino acids that are in the liquids secreted by hornet larvae.
The gel electrolyte is synthesized from a mixture containing the reactive monomers in an organic solution.
10 μL of Power SYBR Green PCR Master Mix, 0.5 μL of a mixture containing the forward and reverse primers (5 μM each), 1 μL of cDNA, and 8.5 μL of H2O were used for each reaction.
10 μL of Power SYBR Green PCR Master Mix, 0.5 μL of a mixture containing the forward and reverse primers (5 μM each), 1 μL of PCR product, and 8.5 μL of H2O were used for each reaction.
Here, the mixture containing the old chewing gum is put into an injection moulding machine.
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