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It is solved by a unique hyperbolic solver valid at each mesh point (pure fluid, pure solid and mixture cell).
The inset shows the percentages of monoclonal (green and no color) and polyclonal (mixture) cell aggregates in the total amount of clusters for cell grown in 4 and 6 mg/mL collagen concentrations.
Not only is carbon dioxide more effective than steam at increasing the mixture cell width, but also its effectiveness increases relative to that of steam with increasing concentrations.
Cells were transfected with 80 nmol/L siRNA duplex mixture (Cell Signaling Biotechnology, Beverly, MA) for 24 h in the presence of lipofectamine RNAiMax (Invitrogen Inc., Carlsbad, CA).
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After removal of the transfection mixture, cells were trypsinised and 2.5 × 104 cells per well were seeded in a 96-well microtitre plate for stimulation experiments.
This application can be valuable in un-mixing biological mixtures, including clinical mixtures, cell cultures, and xenografts [ 24– 29].
Particle mixtures (cell mixtures) and DI water (1× phosphate buffered saline solution) were, respectively, injected into the device through the inlets labeled "particle mixture" and "sheath flow" using syringe pumps (New Era Pump Systems Inc., Farmingdale, NY).
To generate single-mixture cells expressing the α1-PDX inhibitor, the M10/PDX and Colo829/PDX cells were cultured in the presence of 1 µg/ml Pseudomonas exotoxin A [25].
Instead, tumors initiated by luminal cells comprise the same mixture of cell types as tumors initiated by basal cells.
One potential concern with this approach is that primary joint cells are composed of a mixture of cell types.
This result possibly suggests that the NH4+ K+ mixture promotes cell division and NO3- amount promotes cell expansion.
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