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An analytic technique in general use is gas chromatography, which separates the different components of a gaseous mixture by passing the mixture through a long, narrow column of absorbent but porous material.
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Industrial efforts to purify water or natural gas, for example, separate desired compounds from mixtures by passing them through membranes pocked with tiny holes.
This mixture was generated by passing He (40 mL min−1) through a saturator with H2O at 293 K.
The crude product was dissolved in ether and the mixture was trapped by passing through a Silica Plus cartridge.
The tube containing above mixture was bubbled by passing nitrogen gas for 20 min to remove dissolved oxygen completely from polymerization system and then it was sealed.
The mixture is purified by passing it over copper at 720 °C to remove the and the, and then KOH and are used to remove the acids and moisture by sorption.
The mixture was homogenized by passing through a narrow 25-gauge syringe and then incubated at 4 °C with mixing for 1 h.
The bead mixture was analyzed by passing through the detector of a Bio-Plex system (BIO-RAD, Marnes-la-Coquette, France) that identifies the beads based on the fluorescence of the dyes.
Lipid-soluble compounds in the reaction mixture were isolated by passing them through columns of Sephadex G25 Superfine (1 × 3 5 cm in 150 mm Pasteur pipettes, stuffed with glass wool).
The isolated secretary cells were separated from other cells and tissue fragments in the mixture by sequentially passing through a 40 μm and a 30 μm nylon sieves.
Primary cultures of rat cerebellar granule cells at day 2 were treated with 0.5 mM etoposide (VP-16) in serum-free medium for 15 min. The treated cells on 100 mm dishes were lysed with 750 µl of a buffer containing 1% Sarkosyl, 10 mM Tris-HCl (pH 7.5), 10 mM EDTA, and protease inhibitor mixture (Complete Mini, Roche) by passing through a 23G needle 10 times.
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