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∑ i,t is the covariance matrix of the ith Gaussian in the mixture at time t.

ω i,t and μ i,t are an estimate of the weight and the mean value of the ith Gaussian in the mixture at time t, respectively.

It is clearly visible from this figure that only one specie is present in reaction mixture at time 0 min because spectra possess only one peak at 400 nm.

Pseudo-first-order rate constants, k obs, were obtained from the slopes of the plots of (At-A∞) versus time (Figure 8) where At = absorbance of the reaction mixture at time, t(s) after mixing of 2-NH2-Pym solution, and A∞ = absorbance of same after completion of the reaction.

We mixed the effector cells with the target cells at different ratios and measured the luciferase activity from the cell mixture at time points from 24 to 72 h.

Aliquots of 12.5 µl were therefore taken from the reaction mixture at time intervals between 3 to 30 min to ensure linearity of product formation and applied to silica gel 60 F254 TLC plates (Merck, Darmstadt).

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Similar(51)

Cleavage was then initiated by the addition of MgCl2 to a final concentration of 10 mM. 8 µl samples were then withdrawn into stop mixture at timed intervals and processed ready for gel electrophoresis as described above.

The reaction was monitored by measuring the ammonia concentration by the phenol-hypochlorite method [34] in samples, which were removed from the reaction mixtures at time intervals.

Two electrochemical techniques, potentiodynamic polarisation (PP) and electrochemical impedance spectroscopy (EIS), were used to evaluate AA2024-T3 corrosinn in 0.1 M NaCl with the addition of 10−3 M of rare earth chloride mixtures at time periods up to 18 h.

The initial levels of metabolites were obtained by running reaction mixtures at time zero.

31, 32 A sample aliquot was collected from the reaction mixture at serial time points, where the zero time point corresponded with the time when the NADPH regenerating system was added to the reaction mixture.

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