Sentence examples for mixture as template from inspiring English sources
To test for recombination, we performed PCR using either the total DNA or an artificial amplicon mixture as template, together with primers designed to amplify a bi-species PCR recombination product (Figs. 4 and S2).
PCR products of the selected genes were generated using the RT-PCR mixture as template.
A second round of amplification was performed using the initial PCR mixture as template and primers internal to the first amplified sequence (SVC1-2 644 bp upstream and SVC2-3 281 bp downstream of the predicted excision site).
PCR was run using 0.9 μl of the reverse transcription reaction mixture as template, 0.4 mM of gene specific primers, 1 × PCR buffer, 0.25 mM dNTPs, and 1.5 units of Taq DNA polymerase (BioMan, Taipei, Taiwan).
Three-fold serial dilutions were made from this mixture as templates for the calibration curve.
Furthermore, this unique method using the other polycarboxylate and divalent metal ions mixtures as templates can be extended to synthesize similar hollow nanoparticles.
The results showed that the oxidation peak current of Hoechst decreased depending on the number of E. coli cells added to the PCR mixture as the template for amplification, and the sensitivity of the method was as low as a single bacterium.
Using the total DNA mixture as the template, we amplified the intended target region for just two of the eight potential recombination products.
The second round of amplification was performed like the first round with 2 µl of the first-round reaction mixture as the template and primers Linker1 and LJR1, which was identical to the leftmost end of SGI1 (Table 1).
The reaction was carried out in 20 μl total volume containing 500 nM of each primer with 2 μl of the RT reaction mixture as a template.
Following nested PCR using the oligonucleotides Lmo0189NestedAB and Lmo0191NestedCD and the ligation mixture as a template, the resulting fragment was cloned into pLSV101 via BamHI and EcoRI giving rise to pKS0190del.
