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The DNSs for stratified turbulent ignition show that the scales introduced by the mixture and thermal stratifications have a stronger effect on the second and third stage ignitions.
It is shown that only the radial gradient can cause separation of mixture and thermal diffusion caused by radial temperature gradient performs as the main driving force of thermoacoustic separation.
For primer sequences, PCR mixture and thermal cycle programs, see Additional file 5. ExoI-SAP (Amersham Biosciences) was used to purify the PCR products.
The presence of the glmM gene in each strain was established by PCR using primers, the reaction mixture, and thermal cycling [ 19, 20].
The PCR reaction mixture and thermal cycling conditions for the ITS amplicon library were identical to those used to generate the bacterial library except that the 5 μM forward primer (ITS5: 5'-GGAAGTAAAAGTCGTAACAAGG-3') and the 5 μM reverse primer (ITS4: 5'- TCCTCCGCTTATTGATATGC-3') [ 58] were substituted and 35 PCR cycles were used.
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In addition, sensitivity analysis is conducted with respect to the amount of catalyst employed, relative volatilities of reacting mixtures, and thermal condition of feeds.
However, replacing virgin materials in AC mixtures with aged, stiffer binders poses numerous technical challenges in terms of mixture volumetrics and thermal (low temperature) and flexural fatigue cracking resistance.
Depending on the length of soft segment, average functionality of polyol mixtures, mechanical and thermal properties of samples were varied.
However, some others [27 29] show that the thermal conductance mechanism in nanofluids is no different from that in binary solid composites or liquid mixtures, and that thermal conductivity data lie between the well-known effective medium bounds of the Hashin and Shtrikman (H-S) [30].
The density was determined by the pycnometer method; the electrical conductivity using a conductivimeter; the thermal diffusivity by a method based on the analytical solution of heat diffusion equation; the specific heat capacity by a modified method of mixtures; and the thermal conductivity from the knowledge of other properties.
PCR mixtures and the thermal cycler program were as described above.
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