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It has also been found that the UHMWPE/kaolin composites prepared by polymerization have much better tribological properties than the ones prepared by mixing with the same components and are promising self-lubricated material candidates for engineering application.
In all cases eggs were subjected to 2.5% DMSO due to mixing with the same volume of aqueous suspension.
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The tests are based on symmetric cells (electrode/electrolyte/electrode) prepared using Li2CO3 and Na2CO3 mixed with LC or LSCF as composite electrodes, and Gd-doped ceria mixed with the same carbonates as composite electrolyte.
After centrifugation, the supernatant was aspirated and mixed with the same volume of chloroform-isoamyalcohol (24:1) (Amresco) to remove phenol and proteins left through centrifugation.
After the digestion of proteinase K, beads were removed by a magnetic shelf, and samples were mixed with the same volume of phenol-chloroform-isoamyalcohol (25:24:1) (Sigma) to extract DNA.
Four different concrete mixes with the same cement content and different w/c ratios were designed using normal dolomite aggregate, air-cooled slag by-product and two different types of iron ore aggregates.
Samples were withdrawn, mixed with the same volume of 2xSDS-PAGE loading buffer (250 mM Tris-HCl (pH 6.8), 4% (wt/vol) SDS, 30% (vol/vol) glycerol, 10 mM DTT, a trace amount of bromophenol blue) and incubated at 37 °C for 10 min.
The supernatant was mixed with the same volume of ethyl acetate.
bSpore suspension mixed with the same amount of extract was incubated at 28°C for 4 h.
The untreated control soil was mixed with the same volume of deionised water.
(E) Different concentrations of AP21967 were mixed with the same concentration of mKate2-FKBP (1 µmol/L).
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