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The cultures of the two bacterial strains were fixed by mixing with equal volumes of a × 2 fixative solution to give final concentrations of 2.0% w/v paraformaldehyde and 2.5% w/v glutaraldehyde in 50 mM sodium cacodylate buffer (pH 7.4).
Cultures were grown to an OD of 0.5 before mixing with equal volumes of either CaCl2 or DTT.
The wtDR1/HAY308A complexes were incubated for an additional hour with a molar equivalent of soluble SEA or SEH before mixing with equal volumes of SDS-PAGE sample buffer containing 0.2% SDS and incubating for 10 min at room temperature.
Cleavage was analyzed by mixing with equal volume of 2× sample buffer.
Lipoplexes were prepared by gently mixing with equal volumes of NF-κB decoy and liposome solution at a charge ratio of 1.0 2.3.
For western blotting assay, the protein samples were denatured by mixing with equal volume of 2 × sample loading buffer and then boiling at 100 °C for 5 min.
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When sieved and mixed with equal parts of sharp sand/perlite and compost, it makes good seed and potting compost.
Different instrument lines are mixed with equal energy.
Samples were mixed with equal volume of 2% formalin in phosphate-buffered saline for microscopic counts.
Cells incubated with MWCNTs at each time interval were washed twice with PBS before trypsinization, following which cell suspensions were mixed with equal volume of 0.4 % trypan blue.
A 1-mL aliquot of this diluted solution was mixed with equal amounts of DNSA solution and incubated in a boiling water bath for 10 min.
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