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Mixing was continued until a dry precursor was obtained.
The mixing was continued until a uniform color was obtained.
Then, 80%% of water was added and mixing was continued for a further 1 min.
The mixing was continued until the mix was found to be uniform in appearance.
Coarse aggregate was subsequently added to the rotating mixer and mixing was continued for further 2 min.
Finally, the remaining water along with the superplasticizer was poured gradually and mixing was continued for an additional 3 min.
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After an hour, the pH is progressively adjusted to 7.0 using sodium bicarbonate [NaHCO3, simulated intestinal fluids (pancreatin and bile) are added, and mixing is continued for another four hours. The mixture is then centrifuged and settled, and the supernatant analyzed.
To collect immune complexes, 50 µl Protein A/G Agarose-PLUS (Santa Cruz) was added to each tube and slow mixing rotation was continued for 2 hours.
The transfection mixes were replaced with fresh growth media and incubation was continued for 48 hrs.
Mixed breastfeeding is continued breastfeeding up to six months postpartum, supplemented by formula or solids.
As a result, there is continued mixing of phenotypic spring-run fish into the fall-run broodstock.
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