Exact(8)
After mixing vigorously for 15 s, the sample was then centrifuged at 12,000 g for 15 minutes.
L-DOPA (4 mg ml−1) was dissolved in sterile distilled water by mixing vigorously for at least 10 minutes.
After mixing vigorously, the samples were incubated at 65°C for 6+ hrs in a heat block for decrosslinking.
For each injection, 0.2 mg of the immunogen was emulsified with NaCl (0.9 %) and Freund's adjuvant by mixing vigorously.
Microemulsion based organogels (MBGs) were prepared by addition of molten gelatin (14%) maintained at 55°C to the reverse micelles (1 mL) and mixing vigorously till homogenous.
After mixing vigorously for three cycles of 1 min, using a vortexer (Vortex Genie-2, Scientific Industries Inc., Bohemia, NY, USA), the suspension was subjected to centrifugation at 10,000 ×g, at 4°C for 45 min.
To determine GSSG, GSH in samples was derivatized by adding 2.5 μL of 4-vinylpyridine (95%) per 250 μL solution, mixing vigorously for 1 min and incubating samples at room temperature for 30 min. Samples were then centrifuged at 14,000 × g for 5 min and GSSG was measured in supernatants by the procedure described for t-GSH.
Beating means mixing vigorously to get air into the cake.
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